The 26.4 Data Release includes updates to human and model studies, including the Ast10_Communication and MODEL-AD RNAseq Harmonization studies.

Proper acknowledgment of data sources is not only mandatory for users of the AD Knowledge Portal, it also enhances the visibility of your work and elevates recognition of datasets used. All data use must be acknowledged with the following: the AD Knowledge Portal Acknowledgement Statement; and an Acknowledgement Statement specific to the study/dataset used, from the relevant Study page; and a Data Availability statement that includes a direct link to access relevant resources, known as a dataset DOI. For more information and pre-written acknowledgement statements, see Data Use & Acknowledgement.

Human

  • The Ast10_Communication study investigates cell-cell signaling underlying an astrocytic state linked to cognitive decline in Alzheimer’s disease. It combines system-wide computational analysis of new and existing human omics datasets. 

    • This release includes single-nucleus RNA-seq, spatial transcriptomics, and other omics datasets from human samples, together with targeted perturbation data from model systems.

  • The Alzheimer Gut Microbiome Project (AGMP) Alzheimer’s Disease Research Center (ADCR) Study aims to define a role for the gut microbiome and metabolome in Alzheimer’s Disease through a multi-year longitudinal study with ~1000 participants, including those who are cognitively normal (at risk), have mild cognitive impairment (MCI), or have an AD diagnosis. 

    • The release provides UCSD untargeted metabolomics and Nightingale NMR metabolomics data from these participants. 

  • The ROSMAP GAGE-seq study (ROSMAP_GAGE-seq) provides a multimodal single-cell and spatial transcriptomics dataset from postmortem human prefrontal cortex tissue from individuals with Alzheimer’s disease (AD) and age-matched individuals without AD from the ROSMAP cohort. 

    • This release contains raw and processed GAGE-seq data and Xenium PRIME 5K spatial transcriptomics data. GAGE-seq is a technique enabling joint profiling of gene expression and 3D chromatin contacts in the same single cells. 

Models

  • The Jax.IU.Pitt_B6.Klotho Study (Jax.IU.Pitt_B6.Klotho) 

    • This study examines how two human variants of the aging-related gene Klotho (Kl) influence brain gene expression in mice. C57BL/6J mice were engineered via CRISPR/Cas9 to carry either the FC (susceptible) or VS (resistant) human Klotho haplotype, which differ by two amino acid substitutions in exon 2. Whole-brain RNA-Seq was performed on male and female mice at four and 12 months of age to capture age-dependent transcriptomic differences between haplotype carriers. 

  • The MARMO-AD Study

    • This study aims to generate, characterize, and validate the marmoset as a non-human primate model of aging and Alzheimer’s Disease. This release includes new metadata files, new proteomics processed and raw files, new RNAseq and WGS processed and raw files, as well as updates to cognitive and immunoassay data.

  • The Jax.IU.Pitt_LOAD1.PrimaryScreen Study (Jax.IU.Pitt_LOAD1.PrimaryScreen) 

    • The purpose of this project is to provide initial characterization of novel mouse models of late-onset Alzheimer’s disease designed by MODEL-AD and created at The Jackson Laboratory. These new mouse strains express combinations of risk alleles identified from human LOAD data repositories. Here, all mice express APOE4 and Trem2*R47H which has been termed “LOAD1” background. The ten additional genes listed in the table above were all screened on this background, and compared to “LOAD1” control cohorts, for behavioral phenotypes and molecular signatures associated with disease severity. Each strain was aged in cohorts to 4 months and 12 months of age, tested and sampled. 

  • The MODEL-AD RNAseq Harmonization Study (MODEL-AD_RNAseq_Harmonization)