The 26.3 Data Release includes updates to the Columbia University Medical Center’s single nucleus multiomic cohort and the Mayo Clinic Study of Aging

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Human

  • The Columbia University Medical Center’s single nucleus multiomic cohort (CUIMC2_Mult) consisted of 240 participants from the Religious Orders Study and Memory Aging Project (ROSMAP), which is a longitudinal clinical-pathologic cohort of aging and Alzheimer’s disease with participants from two prospective studies of The Religious Order Study (ROS) (N=96) and the Memory and Aging Project (MAP) (N=144). 

    • This release provides raw and processed 10x multiomic data for participants (from postmortem dorsolateral prefrontal cortex tissue). This includes raw sequencing files (FASTQ), cell-type-resolved RDS files of the processed data, and expression matrices, barcode, and feature files.

  • The Mayo Clinic Study of Aging (MCSA) consists of two studies from the Mayo Clinic Study of Aging (MCSA) that include genetic and gene expression data from participants with PAXgene blood collections. Each study provides accompanying diagnosis and demographic information (e.g., age, sex, race). Participants span clinical categories including Alzheimer’s disease (diagnosed using NINCDS-ADRDA criteria), mild cognitive impairment, normal cognition, and other neurologically evaluated conditions. The two studies share five overlapping participants, though gene expression data were generated independently.

    • This release includes gene expression data from MCSA study 2. This release provides independently generated RNA-seq gene expression data from 108 PAXgene blood samples in MCSA Study 2, including raw reads and gene counts processed through a standardized bioinformatics pipeline. Data were generated using Illumina library preparation and sequencing, with quality control filtering (excluding 3 low-quality samples) and normalization via conditional quantile normalization to produce log2-scaled expression values.