The 19.5 Data Release includes an exciting new study provided as part of the AD Portal’s Community Data Contribution Program (CDCP)! The SEA-AD study provides single-nucleus RNAseq, single-nucleus ATACseq, and 10x Multiome data from brain tissue of AD and neurotypical donors. Other updates include differential expression results from the RNAseq Harmonization study and bulk brain and single-cell RNAseq data from Plexin-B1 knockout mice.
This study provides transcriptomic and chromatin accessibility data from single nuclei isolated from brain tissue of 84 aged individuals spanning the full spectrum of Alzheimer’s disease severity and 5 neurotypical adult reference individuals. The Seattle Alzheimer’s Disease Brain Cell Atlas (SEA-AD) consortium includes the Allen Institute for Brain Science, the University of Washington, and Kaiser Permanente Washington Health Research Institute. This release includes:
snATACseq data from the middle temporal gyrus (100 specimens)
snRNAseq data from the middle temporal gyrus (201 specimens) and dorsolateral prefrontal cortex (15 specimens)
Chromium 10x Multiome data from the middle temporal gyrus (28 specimens)
This study is a release of RNA sequencing data from the ROSMAP, MSBB, and MayoRNAseq studies that has been processed using a common workflow. The study is an extension of the rnaSeqReprocessing study, but uses the GRCh38 reference genome for sequence alignment. This release provides:
Filtered, normalized, and residualized gene counts
Differential gene expression analysis results and html reports across all cohorts from the Sageseqr workflow
This study provides bulk brain RNAseq and single-cell RNAseq data from Plxnb1 knockout (KO) mice (C57BL/6J background) which were crossed with APP/PS1 transgenic mice or TAU (TAU-PS19) transgenic mice.