The 19.5 Data Release includes an exciting new study provided as part of the AD Portal’s Community Data Contribution Program (CDCP)! The SEA-AD study provides single-nucleus RNAseq, single-nucleus ATACseq, and 10x Multiome data from brain tissue of AD and neurotypical donors. Other updates include differential expression results from the RNAseq Harmonization study and bulk brain and single-cell RNAseq data from Plexin-B1 knockout mice.

Human

  • The SEA-AD Study

    This study provides transcriptomic and chromatin accessibility data from single nuclei isolated from brain tissue of 84 aged individuals spanning the full spectrum of Alzheimer’s disease severity and 5 neurotypical adult reference individuals. The Seattle Alzheimer’s Disease Brain Cell Atlas (SEA-AD) consortium includes the Allen Institute for Brain Science, the University of Washington, and Kaiser Permanente Washington Health Research Institute. This release includes:

    • snATACseq data from the middle temporal gyrus (100 specimens)
    • snRNAseq data from the middle temporal gyrus (201 specimens) and dorsolateral prefrontal cortex (15 specimens)
    • Chromium 10x Multiome data from the middle temporal gyrus (28 specimens)

  • The RNAseq_Harmonization Study

    This study is a release of RNA sequencing data from the ROSMAP, MSBB, and MayoRNAseq studies that has been processed using a common workflow. The study is an extension of the rnaSeqReprocessing study, but uses the GRCh38 reference genome for sequence alignment. This release provides:

    • Filtered, normalized, and residualized gene counts
    • Differential gene expression analysis results and html reports across all cohorts from the Sageseqr workflow

Models

  • The Plxnb1_KO Study

    This study provides bulk brain RNAseq and single-cell RNAseq data from Plxnb1 knockout (KO) mice (C57BL/6J background) which were crossed with APP/PS1 transgenic mice or TAU (TAU-PS19) transgenic mice.

  • The UCI_5XFAD Study

    This is a study of a familial Alzheimer’s disease mouse model expressing mutant human APP and PS1. This release provides:

    • Updated individual and biospecimen metadata
    • Updated Immunoassay data 
    • Added RNAseq data (FASTQ) for missing specimens

  • The UCI_3xTg-AD Study

    This study provides transcriptomic data from the hippocampus and cortex of mice expressing three mutations of APP, MAPT, and PSEN1. This release provides:

    • Updated Immunoassay (ELISA) data