Data Release 25.2 includes updates to the SEA-AD Study and CUMC Compounds Microglia study.

Human

  • The SEA-AD Study

    This study provides transcriptomic and chromatin accessibility data from single nuclei isolated from multiple brain tissues of 84 aged individuals spanning the full spectrum of Alzheimer’s disease severity and 5 neurotypical adult reference individuals. The Seattle Alzheimer’s Disease Brain Cell Atlas (SEA-AD) consortium includes the Allen Institute for Brain Science, the University of Washington, and Kaiser Permanente Washington Health Research Institute. 

    • This release provides 10x Multiome and 10x single-cell RNA sequencing data sourced from hippocampus, lateral entorhinal cortex, and inferior temporal gyrus samples collected from 96 total individuals. It also provides updated clinical metadata and new Multiome and single-cell RNA sequencing assay metadata.

Models

  • The CUMC Compounds Microglia

    This study uses different model systems including HMC3 cells, iPSC-derived microglia and cerebral organoids to recapitulate human microglial subtypes  in vitro, enabling functional characterization and providing a foundation for modulating human microglia in vivo.

    • This release provides single-cell RNA sequencing FASTQ and UMI count matrix files. It also provides clinical metadata and assay metadata for 24 samples.